Q1: Is there data for this product in CRISPR-Cas9 RNP gene editing? What about dosage information?
Cas9; protein,; sgRNA,; Protein transfection reagents, mass ratios, are respectively 2; 1,; and4; μ g/mL, and you can optimize it on this basis yourself
Q2: What are the possible reasons for cell death in the control group? Was the growth medium not removed before transfection? Having serum and antibiotics during transfection – is this the cause of cell death? Or does it only affect the transfection reagent's efficacy?
It contains serum and double antibodies, which will not cause cell death and will not affect the transfection effect.
Pay attention to exploring the optimal concentration. If the concentration of the transfection reagent is too high, the cells will definitely die.
In addition, it should be noted that the main culture medium accounts for the majority of the cells; otherwise, without waiting for osmotic conditions, it will also cause cell death.
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