Product Information

• 产品货号 B0099
• 别名 ACTB; Actin; cytoplasmic 1; Beta-actin; Actin β;β-Actin;Beta actin
• 产品名称 Actin beta Rabbit Polyclonal Antibody
• 类别 Antigen Antibody
• 基因名称 ACTB
• 蛋白名称 Actin cytoplasmic 1
• 推荐应用 WB,IHC-P,IF-ICC
• 反应种属 Human;Mouse;Rat;Chicken;Globefish;Bovine;Hamster;Pig;Ovine;Cat;Pig;Dog
• 存储缓冲液 PBS, pH 7.4, containing 0.5%BSA, 0.02% New type preservative N as Preservative and 50% Glycerol.
• Human Gene ID 60
• Mouse Gene ID 11461
• Mouse Swissprot No. P60710
• Rat Gene ID 81822
• 免疫原 Synthesized peptide derived from the N-terminal region of human Actin β. AA range: 1-80
• 特异性 Actin β Polyclonal Antibody detects endogenous levels of Actin β protein.
• 稀释度 IF-ICC 1:50-200, WB 1:2000-1:10000. IHC-P 1:100-1:300. ELISA 1:20000. Not yet tested in other applications.
• 参考分子量 42kD
• 运输及保存条件 -15°C to -25°C/1 year(Do not lower than -25°C)
• 宿主 Rabbit
• 同种型 IgG
• 表达 B-cell lymphoma,Brain,Cajal-Retzius cell,Eye,Fetal brain cortex,Foreskin,Hepatocellular car

Picture

	Immunofluorescence analysis of human-uterus tissue. 1,Actin β Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
	Immunofluorescence analysis of rat-lung tissue. 1,Actin β Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
	Western Blot analysis of 1,hela 2,A549 3,HEPG2 4,Mouse-brain 5,Mouse-lung 6,Mouse-liver 7,Rat-brain 8,Rat-lung 9,Rat-liver cells using primary antibody diluted at 1:1000(4°C overnight). Secondary antibody:Goat Anti-rabbit IgG IRDye 800( diluted at 1:5000, 25°C, 1 hour). Cell lysate was extracted by Minute™ Plasma Membrane Protein Isolation and Cell Fractionation Kit.
	Immunohistochemical analysis of paraffin-embedded Human-uterus tissue. 1,Actin β Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
	Immunohistochemical analysis of paraffin-embedded Human-uterus-cancer tissue. 1,Actin β Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
	Immunohistochemical analysis of paraffin-embedded Human-colon tissue. 1,Actin β Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
	Immunohistochemical analysis of paraffin-embedded Human-liver-cancer tissue. 1,Actin β Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
	Immunohistochemical analysis of paraffin-embedded Human-uterus-cancer tissue. 1,Actin β Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
	Immunohistochemical analysis of paraffin-embedded Human-stomach-cancer tissue. 1,Actin β Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
	Immunohistochemical analysis of paraffin-embedded Rat-kidney tissue. 1,Actin β Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
	Immunohistochemical analysis of paraffin-embedded Mouse-heart tissue. 1,Actin β Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
	Immunohistochemical analysis of paraffin-embedded Mouse-lung tissue. 1,Actin β Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
	Immunohistochemical analysis of paraffin-embedded Mouse-spleen tissue. 1,Actin β Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
	Immunohistochemical analysis of paraffin-embedded Human testis. 1, Antibody was diluted at 1:100(4° overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).
	Aikeqing, a kidney- and spleen-tonifying compound Chinese medicine granule, prevented ovariectomy-induced bone loss in rats via the suppression of osteoclastogenesis. BIOMEDICINE & PHARMACOTHERAPY Xiao-Ling Shen WB Rat，Mouse left femurs bone marrow macrophages (BMMs)

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