The sugar groups of HRP are oxidized into aldehyde groups by periodic acid, which can react with the amino groups of proteins

Some measures can help improve the labeling effect: 1) Enhance the antibody to be labeled, such as using antigen affinity chromatography for purification...

The sugar groups of HRP are oxidized into aldehyde groups by periodic acid, which can react with the amino groups of proteins

Some measures can help improve the labeling effect :1) Increase the antibody to be labeled, such as using antigen affinity chromatography for purification; 2) Pay attention to the mass ratio of antibody to HRP, it is recommended to be between 1:1 and 1:4; 3) Extend the reaction time between HRP and antibody At room temperature, it can last for 4 hours, or 4 hours overnight. If time permits, 4 hours overnight is preferred. 4) Throughout the experiment, pay attention to keeping HRP away from light. 5) The concentration of the antibody to be labeled should be greater than 4mg/ml before labeling. 6) Reduce the reaction volume during antibody labeling to ensure that the concentrations of antibodies and HRP reach above 2mg/ml. 7) Use 0.5ML ultrafiltration tubes; 4ml ones are not suitable for micro-labeling. Therefore, it is advisable to choose ultrafiltration tubes with smaller volumes to minimize losses. 8) Control the reaction volume during HRP and antibody labeling. The ideal volume is 250 to 500 μ L

The storage stability is mainly determined by the source antibody, and the labeling has little effect on the stability of the antibody. Antibodies are generally stored in glycerol to avoid repeated freeze-thaw cycles and adsorption losses, and protective agents such as BSA are added. The specific storage time requires a separate storage stability test for each antibody. The common storage period is 1 to 10 years.