Q1: Question about recycling uses of Soluble TMB single component (HRP substrate)
Question: For the TMB substrate of Boao Long, we are often confused about whether to recycle the leftovers after pouring them out. So, I would like to ask how long it should be kept after pouring to be suitable for recycling and reuse next time? Reply: If it is recycled, it will be contaminated. When dumping, it can be accurately measured by weighing. Pour only as much as needed.
Q2: Question about recycling uses
Recycling would cause pollution. When dumping, it can be accurately measured by weighing. Just dump as much as needed
Q3: Can it continue to be used if accidentally frozen?
Although this product can still be used after being frozen, it will have a certain impact on the product's usage effect. If it is accidentally frozen, shake well after thawing and then use it. It is recommended to conduct a test experiment to determine the usage parameters
Q4: The color development is very fast. Any suggestions to slow it down?
Here are three suggestions: 1. Reduce the amount of antibody/antigen coated on the ELisa plate; 2. Reduce the amount of samples used for testing; 3. Dilute with ultrapure water containing 0.01% hydrogen peroxide before use (this solution is generally not recommended as dilution may cause product instability. The use of diluted products is not covered by the company's warranty).
Q5: Does the Soluble TMB Single Component Substrate contain urea hydrogen peroxide?
no
Q6: How to choose between soluble and precipitable TMB?
TMB is widely used due to its high sensitivity and non-carcinogenicity. When the single-component TMB peroxidase substrate reacts with horseradish peroxidase linker, a deep blue product is produced, which is suitable for both quantitative and qualitative enzyme-linked immunosorbent assay (ELISA) analysis. It is further divided into soluble type and precipitated type