ELISA kit for the identification of mouse monoclonal antibodies Ig/subclasses (including light chains) This kit uses a dual-antibody sandwich method to identify classes and subclasses of monoclonal antibodies or specifically affinity-purified monoclonal antibodies in mouse lymphocyte hybridoma culture supernatants (IgG1\IgG2a\IgG2b\IgG3\IgM\IgA\Kappa\Lambda). The secondary antibody coated microplate with a common site of mouse antibody was used to bind to the mouse antibody in the added culture supernatant, and then HRP-labeled anti-mouse antibodies of various types and subclasses were added to react separately, and finally the TMB substrate system was used to develop color and terminate with dilute sulfuric acid, and then the absorbance was detected by a microplate reader to determine the class or subclass of the tested monoclonal antibody. With its extremely high resolution, this kit is a reliable tool for identifying mouse monoclonal antibody classes and subclasses. Scope of use: It is used for the identification of Ig and subclasses of mouse monoclonal antibodies in culture supernatant and related contents. Product features: High sensitivity, high specificity, and easy to determine results. Of course, if you feel that you can prepare many reagents yourself, you can choose a concentrated secondary antibody set (link) or a ready-to-use secondary antibody kit (link).