Product Information

• 产品货号 BD-PB0656
• 别名 PDHE1-A type I; Pyruvate dehydrogenase E1 component subunit alpha, somatic form, mitochondrial; PHE1A, PDHA1;pdha1a;pdha1b
• 产品名称 Zebrafish PDHA1 Rabbit Polyclonal Antibody (Center)
• 类别 Antigen Antibody
• 推荐应用 WB
• 反应种属 Zebrafish
• 特异性 This Zebrafish PDHA1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 233~263 amino acids from the Central region of Zebrafish PDHA1.
• 稀释度 WB 1:1000
• 预测分子量 44kDa
• 同种型 Rabbit Ig
• 背景介绍 The pyruvate dehydrogenase (PDH) complex is a nuclear-encoded mitochondrial multienzyme complex that catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2), and provides the primary link between glycolysis and the tricarboxylic acid (TCA) cycle. The PDH complex is composed of multiple copies of three enzymatic components: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydrogenase (E3). The E1 enzyme is a heterotetramer of two alpha and two beta subunits. This gene encodes the E1 alpha 1 subunit containing the E1 active site, and plays a key role in the function of the PDH complex. Mutations in this gene are associated with pyruvate dehydrogenase E1-alpha deficiency and X-linked Leigh syndrome.
• 纯化 Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Picture

All lanes : Anti-PDHA1 Antibody (Center) at 1:1000 dilution Lane 1: Zebrafish lysate Lane 2: ZF4 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 44 kDa Blocking/Dilution buffer: 5% NFDM/TBST.

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