Product Information

• 产品货号 BD-PB0666
• 别名 Non-structural protein 6, ns6, Accessory protein 6, Protein X3
• 产品名称 SARS virus PUP3 Antibody (C-term)
• 类别 Pathogenic microorganism antigen-antibody pseudovirus
• 反应种属 SARS
• Human Swissprot No. P59634
• other Gene ID NP_828856.1
• 特异性 This SARS virus PUP3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of SARS virus PUP3.
• 预测分子量 8kDa
• 同种型 Rabbit Ig
• 背景介绍 An outbreak of atypical pneumonia, referred to as severe acute respiratory syndrome (SARS) and first identified in Guangdong Province, China, has spread to several countries. The severity of this disease is such that the mortality rate appears to be ~3 to 6%. A number of laboratories worldwidehave undertaken the identification of the causative agent. The National Microbiology Laboratory in Canada obtained the Tor2 isolate from a patient in Toronto, and succeeded in growing a coronavirus-like agent in African Green Monkey Kidney (Vero E6) cells. This coronavirus has been named publicly by the World Health Organization and member laboratories as ?SARS virus? The SARS membrane proteins, including the major proteins S (Spike) and M (Membrane), are inserted into the endoplasmic reticulum Golgi intermediate compartment (ERGIC) while full length replicated RNA (+ strands) assemble with the N (nucleocapsid) protein. The virus then migrates through the Golgi complex and eventually exits the cell, likely by exocytosis. The site of viral attachment to the host cell resides within the S protein. Oligomeric spike (S) glycoproteins extend from SARS membranes. These integral membrane proteins assemble within the endoplasmic reticulum of infected cells and are subsequently endoproteolyzed in the Golgi, generating noncovalently associated S1 and S2 fragments. Once on the surface of infected cells and virions, peripheral S1 fragments bind carcinoembryonic antigen-related cell adhesion molecule (CEACAM) receptors, and this triggers membrane fusion reactions mediated by integral membrane S2 fragments.
• 细胞定位 Host endoplasmic reticulum membrane. Host Golgi apparatus membrane. Host cytoplasm. Note=Localizes to virus- induced vesicular structures called double membrane vesicles
• 纯化 Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

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