Product Information

• 产品货号 BF04201
• 产品名称 Recombinant SARS-CoV-2 RBD Protein, mFc, His
• 类别 Pathogenic microorganism antigen-antibody pseudovirus
• 浓度 Approximately 1-2 mg/ml (subject to label)
• 存储缓冲液 1、10mm Sodium acetate, 270mm sucrose, 0.01% TWEEN 20, PH6.8；2、PBS, PH7.0
• 参考分子量 51.6 kDa
• 性状 液体
• 宿主 Expression System 293F cells
• property Recombinant Protein, mouse Fc tag, His tag
• 纯化 ≥95 % by SDS-PAGE

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Product Details

Product Name    Recombinant SARS-CoV-2 RBD Protein, mFc, His
 

Catalog No.                          BF04201
Expression System               293F cells
Size                                      1mg-2mg/ml（Subject to product label）
Protein Type                         Recombinant Protein, mouse Fc tag, His tag Calculated MW     51.6 kDa
Form                                     liquid
Purification method              Affinity purification chromatography
Purity                                    ≥95 % by SDS-PAGE
Buffer         1、10mm Sodium acetate, 270mm sucrose, 0.01% TWEEN 20, PH6.8
           2、PBS, PH7.0
Storage                                Store at -20℃/-80℃. Avoid freeze/thaw cycles
Note                                    Centrifuge before opening to ensure vial contents are completely collected


SDS-PAGE
   
Recombinant SARS-CoV-2 RBD mFc tag, His tag, on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-ELISA
Binding of BF04201 to BF04204 (200ng/well)

BF04201 Con.(ug/ml)
BF04201 can bind the immobilized anti-RBD antibody (BF04204, human IgG1 antibody) at an EC50 of 0.19ug/ml.
Binding of BF04201 to ACE2 (200ng/well)

BF04201 Con.(ug/ml)
BF04201 can bind the immobilized ACE2 (His tag) at an EC50 of 0.11ug/ml.