Dextrin MBP Protein Purification Chromatography Media (6FF) Preloaded Column HiCap Dextrin 6FF

GoodsCode: BDTL0015-15
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  • 1×5ml
    1400.00
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Product Information

  • 产品货号 BDTL0015-15
  • 产品名称 Dextrin MBP Protein Purification Chromatography Media (6FF) Preloaded Column HiCap Dextrin 6FF
  • 类别 Purification reagent

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Product Details

产品名 Dextrin MBP蛋白纯化层析介质(6FF)预装柱
货号 BDTL0015-11 BDTL0015-51 BDTL0015-15 BDTL0015-55 BDTL0015-3115
规格 1*1ml 5×1ml 1×5ml 5×5ml 3×1ml+1×5ml
基质 高度交联的6%琼脂糖微球
配体 糊精
载量 >10 mg MBP蛋白(80 kDa)/ml介质
粒径 (um) 45-165
最大流速 0.3 MPa, 3 bar
pH 稳定范围 3.0-12.0
储存缓冲液 20% 乙醇
储存温度 4-8℃

Dextrin MBP蛋白纯化层析介质(6FF)是一种纯化带有麦芽糖结合蛋白(MBP)标签蛋白的亲和层析介质。MBP可促进连接蛋白的正确折叠,增加在细菌中过量表达的融合蛋白的溶解性,尤其是真核蛋白。本层析介质可以一步纯化MBP融合蛋白,结合的融合蛋白可以用10mM麦芽糖进行温和洗脱,保护了标签蛋白的活性。如果要去除MBP融合部分可用位点特异性蛋白酶切除。

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