Under normal circumstances, there is a little sediment in the Freesier complete adjuvant because of mycobacteria. Just mix it thoroughly before use. The 10ml specification is relatively small, so it's not very obvious. The large package will be more obvious. Actually, both are available

The difference between complete adjuvants and incomplete adjuvants is that complete adjuvants contain mycobacteria that are inactivated by ultrasound combined with water bath heating

Neither complete nor incomplete adjuvants contain animal-derived components, except that complete adjuvants have BCG vaccine components

Adjuvant composition issue: Freund's complete adjuvant is composed of an oil phase (generally mineral oil) and an aqueous phase (generally physiological saline). If the ratio of these two phases is incorrect or one of them is contaminated, it may lead to emulsification failure. 1. Temperature issue: Emulsification is a temperature-sensitive process. When preparing Freund's complete adjuvant, it is necessary to ensure that it is carried out at an appropriate temperature, usually at room temperature or slightly above room temperature. Both too low and too high temperatures may affect the emulsification effect. 2. Stirring force: During the emulsification process, sufficient stirring force is required to ensure thorough mixing of the oil phase and the water phase. If the stirring is not thorough enough, the emulsification effect may not be good. Stirring time: Emulsification requires a certain amount of time to ensure thorough mixing. Both too short and too long stirring times may affect the quality of emulsification. 3. Adjuvant aging: The quality of Freund's complete adjuvant will decline over time. If the adjuvant is too old, the emulsifier in it may have lost its activity, resulting in poor emulsification effect. 4. Material quality: Using low-quality or contaminated oil or water phases may lead to emulsification failure. If emulsification failure is encountered during the preparation of Frexfeld's complete adjuvant, attempts can be made to optimize the above factors, such as adjusting the ratio, temperature, and stirring conditions. 5. Alter the usage ratio of antigen adjuvant: Increase from adjuvant to antigen 1:1 to 1:2, thereby increasing the amount of antigen used. If conditions permit, a direct immune test can be conducted to see if the requirements are met. In addition, check all the materials used to ensure their quality is good. During the experiment, pay attention to keeping the operation clean and standardized to avoid the introduction of external contamination. If the problem persists, it may be necessary to prepare the adjuvant again or consider using other appropriate adjuvants. Note: Adding antigens containing SDS or organic substances will affect the emulsification effect. Note: Replace the QuickAntibody series of water-based adjuvants without emulsification, eliminating the trouble of emulsification.

Sure. Store it at 4 degrees Celsius. When you need it the next day, emulsify it simply

Freund's complete adjuvant has no concentration parameter. The content of BCG vaccine that can be provided is less than 10mg/ml, and the specific value is not disclosed to the public.