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Q1: Can the QuickAntibody adjuvants for mice and rabbits be mixed? Can the mouse adjuvant be used for rabbits?

Rabbit adjuvants and mouse adjuvants should not be mixed. They are species-specific and it is not recommended to use them together

Q2: Can whole bacteria be used for immunization?

The surface of bacteria is all polysaccharides, making it difficult to obtain protein epitope antibodies. Polysaccharide epitope antibodies have a high titer. It can be attempted to immunize 1-5 micrograms after quantification by BCA

Q3: Why are most of my antibodies IgM?

Glycoprotein immunogens, such as CA125, are highly prone to generating IgM and difficult to obtain IgG

Q4: Choice of antigen solvent.

Q5: 佐剂适用物种

目前QuickAntibody免疫过：小鼠、大鼠、兔子、豚鼠、山羊、羊驼、鸡、猴子等多种物种，效果均不错。佐剂本身是多种佐剂的混合物，而不是单一佐剂。

物种	佐剂类型	抗原用量ug	抗原体积ul	佐剂体积ul
小鼠	QuickAntibody-Mouse	5~40ug	50	50
大鼠、豚鼠	QuickAntibody-Mouse	20~50ug	70	70
兔子、鸡	QuickAntibody-Rabbit	50~100ug	100	100
山羊、羊驼	QuickAntibody-Rabbit	200~500ug	300~500	300~500
猴子	QuickAntibody-Rabbit	200ug	200~300	200~300

Q6: Are there weight requirements for rabbits?

There is no weight requirement. Currently, when our company immunizes rabbits, we use healthy 6-week-old New Zealand white rabbits (with bright eyes and clean butts, weighing about 2 kilograms).

Q7: For booster immunization, can I use the immunogen plus your Quick Antibody adjuvant? The immunogen is cells, not processed protein.

The main function of impulse immunity is to activate spleen cells to a relatively high extent, which has a significant impact on the ability of hybridoma cells to secrete antibodies after fusion. Therefore, direct antigen impulse immunity is still recommended. Moreover, cells themselves as immunogens are likely to have the problem of low titer, and their components are rather diverse. The target epitope protein may not necessarily be the dominant immunogen. Previously, General Manager Yan said that taking on similar technical services for preparing antibodies through cellular immunity basically could not meet the experimental expectations.

Q8: Can it be used in parallel with aluminum adjuvant?

They can be used in combination. Any immune method can produce the effects of cellular immunity and humoral immunity.

Q9: After one immunization with Freund's adjuvant, can the Quick Antibody adjuvant be used for subsequent immunizations?

Sure. The functions of different adjuvants are similar, and different adjuvants can be tried to be used alternately

Q10: 兔8w佐剂免疫一只兔子的抗体产量

我们这边兔多抗项目，有两种纯化方式：（1）抗原亲和纯化一般纯化10ml抗血清能获得1~3mg纯化后抗体，一只兔子能取50ml抗血清，这样总量应该在5~15mg左右；（2）ProiIenA/G纯化纯化20ml抗血清应该能拿到100~150mg左右纯化后抗体。一般客户采用抗原亲和纯化的多一些，以前一个数据为主。（ps:这些是弗式佐剂免疫的结果）

Q11: Can it be stored at -20°C?

Our adjuvant components are very stable. We have conducted a one-month test at 50℃ and found no effect. However, it cannot be frozen, as freezing can have a significant impact on the immune effect. For general storage, it can be placed once near the door of a 4℃ refrigerator, not at the innermost part. Some refrigerators have poor temperature control, and placing it at the innermost part will keep the temperature below 0℃.

Q12: Why is the water adjuvant more expensive than Freund's adjuvant?

Just looking at the price, it's a bit expensive, but it saves time cost Save one month's time cost and the cost of raising mice 2. Save the amount of antigen used, only 1/5 to 1/10 of the original. The price of 1mg of natural antigen can reach tens of thousands of yuan. Our adjuvant components are very stable. We have conducted a one-month test at 50℃ and found no effect. However, it cannot be frozen, as freezing can have a significant impact on the immune effect. For general storage, it can be placed once near the door of a 4℃ refrigerator, not at the innermost part. Some refrigerators have poor temperature control, and placing it at the innermost part will keep the temperature below 0℃. Sure. The functions of different adjuvants are similar, and different adjuvants can be tried to be used alternately

Q13: Differences between adjuvants, how to choose, can they be mixed?

The five-week adjuvant for mice was the first to come out. Based on the five-week adjuvant for mice, the three-week adjuvant and the two-week adjuvant were optimized. Their main components were the same, but the ratios were slightly different. Therefore, it is generally not recommended to mix them. There are significant differences in adjuvants between rabbits and mice Mouse adjuvants and rabbit adjuvants are not recommended to be mixed. One of the main components of adjuvants has species selectivity. Mouse adjuvants themselves can be mixed, but if there is enough time, it is not recommended to mix them.

Q14: Although the antibody titer meets requirements, why is the mouse spleen not enlarged?

This is normal. Traditional adjuvants contain BCG vaccine. BCG vaccine is a strong immunogen. Many B cells in the spleen target BCG vaccine, so they are larger

Q15: Unsatisfactory titer after 2 immunizations.

For general antigens with weak immunogenicity or for customers using our product for the first time, the titer may not increase after two doses. At this point, a third dose can be administered 14 days after the second dose, which usually leads to an increase of an order of magnitude. However, the maximum number of doses is four. If the titer still does not increase after four doses, it is generally recommended to re-administer or modify the antigen. The more common ones are small molecule antigens such as polypeptides and small molecules. It is generally recommended to increase the number of aa peptides to more than 20, which can effectively enhance the antibody titer.

Q16: Composition of the adjuvant.

This adjuvant is a confidential formula of our company. The specific ingredients are not available for disclosure. An English description of the principle can be provided QuickAntibody is a proprietary novel adjuvant designed specifically for the preparation of monoclonal as well as polyclonal antibodies in mice. It is formulated with PAMPs (pathogen associated molecular patterns) and cationic polymers to activate cytosolic PRRs (pathogen recognition receptors)."

Q17: Adjuvant precipitation.

The precipitate is the effective component of our adjuvant and cannot be removed by centrifugation. Last year, there was less precipitate in the adjuvant, but this year there is more. The adjuvant was updated and replaced in November 2021. The new adjuvant has been verified for more than five years and has better effects.

Q18: Can it be used for vaccine development?

Some of our adjuvant components are used for vaccine research and development, but the cooperation will not be completed until the second half of 2022. Our adjuvant components are used for the development of human vaccine adjuvants. Generally, customers cannot afford to use them for animal vaccines, except for pets and special animals.

Q19: Can it be used for immunization with nucleic acid plasmids or RNA?

For nucleic acid immunization, our adjuvant is not applicable. Liposomes can be used instead.

Q20: Can it be used for cellular or viral antigens, i.e., non-protein antigens?

Our adjuvants have been tested through tens of thousands of antigen immunization experiments. Currently, the types of antigens available for immunization include cells, viruses, proteins, polypeptides, polysaccharides, lipids, heavy metals, antibiotics, pesticides, drugs, and food safety compounds, all of which have achieved excellent results. We have a wealth of industrial customer cases and hundreds of literature articles to support this.