no

a.肿瘤细胞系（143B、A549、MDA-MB-231、MDA231-LM2、4T1、H441、ADTC5、Hep3B、HepG2、AML12、HeLa）b.正常细胞系（HEK293、L929、MC3T3-E1、MLO-Y4、HaCaT、HKF、APRE-19、RAW 264.7）c.原代细胞（chondrocyte）d.干细胞（hMSC）悬浮细胞SK-MEL-1（有很好的转染效率）

The structure of the key raw material polymer is consistent, but the production process of the "RNA in vivo transfection reagent" has been improved, with increased purity and lower endotoxin to meet the requirements of in vivo experiments.

Our RNA transfection reagents (KX0110049&KX0110050) are only suitable for RNA(including siRNA, miRNA, and recombinant RNA, but excluding mRNA). Through experiments, it has been found that they are not suitable for DNA transfection. Therefore, it is also not suitable for co-transfection of RNA and DNA. In addition, currently, most of the reagents available on the market are separate for DNA and RNA transfection reagents, and they are generally not interchangeable.

The dilution buffer recommended here is mainly to maintain an isotonic environment, keep the osmotic balance inside and outside the cells, and help maintain normal cell function and physiological state. After the non-isotonic solution is added, the smaller the volume, the lower the risk of damage. However, the final effect can only be further analyzed and judged by the customer based on the actual situation

It is recommended to use the isotonic solution tested in the laboratory (5% glucose solution prepared with DEPC sterile water) to prepare the RNA and transfection reagent complex. If other solutions need to be tried, DEPC sterile water or serum-free medium can be tested. However, the results were more stable when 5% glucose solution (w/v, prepared with sterile DEPC water) was selected.

The RNA purification methods are all similar and there is no difference

When customers generally need RNA transfection reagents, it is for the study of RNA functions. Usually, single pure RNA molecules are used, and the main sources are prepared by chemical synthesis, semi-chemical synthesis or biosynthesis methods. The purification methods commonly used are PAGE or HPLC. Total RNA is obtained from biological samples, and a specific RNA molecule (a single RNA) needs to be further isolated. The amount of RNA extracted biologically is small and the types are numerous. The subsequent separation and purification methods need to be very complex and are generally not suitable for RNA transfection experiments.

a. Tumor cell lines (143B, A549, MDA-MB-231, MDA231-LM2, 4T1, H441, ADTC5, Hep3B, HepG2, AML12, HeLa) b. Normal cell lines (HEK293, L929, MC3T3-E1, MLO-Y4, HaCaT, HKF, APRE-19, RAW 264.7) c. Primary cells (chondrocyte) d. Stem cells (hMSC) suspension cells SK-MEL-1 (with excellent transfection efficiency)