Q1: If saline is not available, can basal medium be used as a diluent?
We prioritize the optimization with normal saline, so we recommend normal saline as the diluent first. Next comes PBS
Q2: Is your transfection reagent GMP grade?
no
Q3: PEI转染试剂和lipo3000转染A549细胞哪个好一点。
PEI 转染试剂转不了A549，推荐使用我们的增强型转染试剂（KX0110042）
Q4: Will mycoplasma contamination prevent transfection?
It will affect the cell state, and there are many cell deaths after transfection
Q5: What should I do if 293T cells float when changing medium 24 hours after adding the virus for titer measurement?
When adding the liquid, try to add it along the wall of the culture dish as much as possible, and add it slowly, because 293t itself is adherent cells
Q6: Which is better for transfecting A549 cells, PEI transfection reagent or lipo3000?
PEI transfection reagent cannot transfect A549. We can recommend our enhanced transfection reagent.
Q7: Will mycoplasma contamination prevent transfection?
It will affect the cell state, and there are many cell deaths after transfection
Q8: What should I do if 293T cells float when changing medium 24 hours after adding the virus for titer measurement?
When adding liquid, try to add along the wall of the culture dish as much as possible and do it slowly. This is because when packaging lentivirus, the 293T cells take a long time to culture, and there may be a situation where they do not adhere firmly
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