The color difference is due to the fact that both our cryopreservation solution and the addition factor contain phenol red. During the packaging process, the liquid reacts with co2 in the atmosphere to become acidic, which will cause a color change. However, it has no impact on the product quality. Phenol red is an acid-base indicator

每孔大概是200ul，96孔，大概是20ml，按1:10比例，大概使用杂交瘤添加因子2ml，加上不同客户用量不同，大概1个96孔板大概用杂交瘤添加因子1-2ml

It needs to be added continuously.

Each well is approximately 200 μ l, and for 96 Wells, it's about 20 mL. At a ratio of 1:10, approximately 2ml of hybridoma additive factor is used. Considering that the usage varies among different customers, approximately 1-2ml of hybridoma additive factor is needed for one 96-well plate