抗体IgM都带有HIS标签，IgG都不带

从人的血液里获得抗体的序列信息，B细胞流式分选，单细胞RT-pcr扩增，获得抗体序列，构建到表达载体，并通过293细胞瞬转表达，获得人源化抗体

All IgM antibodies carry the HIS tag, while IgG antibodies do not

The sequence information of antibodies was obtained from human blood, sorted by B-cell flow cytometry, amplified by single-cell RT-pcr to obtain antibody sequences, constructed into expression vectors, and transiently expressed in 293 cells to obtain humanized antibodies