The maximum flow rate for a 1ml pre-packed column is 1ml/min. The recommended flow rate for sample loading is 0.2-0.3ml/min. The recommended flow rate for the equilibrium washing and elution steps is 0.5-1ml/min. The maximum flow rate for a 5ml pre-packed column is 5ml/min. The recommended flow rate for sample loading is 0.5-1ml/min. The recommended flow rate for equilibrium impurity washing and elution is 2-3ml/min