Q1: For G-column purification, is there a pH requirement for the 6M guanidine hydrochloride solution used to wash the column?
Select guanidine hydrochloride solution with a pH of 8.7
Q2: I didn't enrich antibodies in my purification. Can the flow-through, wash, and eluate be passed through the column again?
Sure, but if the antibody content in the sample itself is not high, the amount of antibodies obtained from re-purification may not be large either, and even less due to loss
Q3: What is the centrifugal force for the resin?
When centrifuging the agarose matrix fillers of Boaolong Company with a centrifugal force of 5000-8000g, this centrifugal force condition can be used
Q4: What is the maximum sample volume that can be added to the 3ml gravity pre-packed column set at one time?
The total volume of the columns used in all gravity pre-packed column sets of Boao Long is 12ml. After removing 3ml of packing material, approximately 5 to 8ml of sample can be added at one time. Please note not to exceed the total load of the packing material contained.