Q1: I didn't enrich antibodies in my purification. Can the flow-through, wash, and eluate be passed through the column again?
Sure, but if the antibody content in the sample itself is not high, the amount of antibodies obtained from re-purification may not be large either, and even less due to loss
Q2: What is the centrifugal force for the resin?
When centrifuging the agarose matrix fillers of Boaolong Company with a centrifugal force of 5000-8000g, this centrifugal force condition can be used
Q3: What is the maximum sample volume that can be added to the 3ml gravity pre-packed column set at one time?
The total volume of the columns used in all gravity pre-packed column sets of Boao Long is 12ml. After removing 3ml of packing material, approximately 5 to 8ml of sample can be added at one time. Please note not to exceed the total load of the packing material contained.
Q4: How many times can this product be reused? What is the protein recovery rate?
The number of reuses is related to the impurity content in the sample. Generally, it is fine to reuse it 8 to 10 times. If it is well maintained, the number of reuses will be even higher. The protein recovery rate is generally 90-95%