Q1: Is there information on the NaOH concentration used in the alkali resistance test of recombinant alkali-resistant protein A, the number of elution cycles, and the dynamic binding capacity after multiple cycles?
Without the above-mentioned relevant experimental data, our test was as follows: Soaking in 0.5M sodium hydroxide for 8-12 hours had no effect on the protein. The evaluation index was the comparison of protein purity before and after soaking, and the detection method was SDS-PAGE.
Q2: Is there information on the NaOH concentration used in the alkali resistance test of recombinant alkali-resistant protein A, the number of elution cycles, and the dynamic binding capacity after multiple cycles?
Without the above-mentioned relevant experimental data, our test was as follows: Soaking in 0.5M sodium hydroxide for 8-12 hours had no effect on the protein. The evaluation index was the comparison of protein purity before and after soaking, and the detection method was SDS-PAGE.