Q1: Can it recognize both the full-length and p35?
yes
Q2: What is the principle of the desalting column?
Its working principle mainly utilizes the function of molecular sieves to separate substances based on the different molecular sizes of the substances to be separated. After the sample enters the centrifugal desalination column, molecules larger than the maximum pore size of the matrix cannot enter the matrix gel. They move along the gaps between the matrix gel particles with the flowing solution at a relatively fast downward speed and are eluted from the column first. Molecules smaller than the pore size of the matrix will continuously enter the interior of the matrix gel pore size. Therefore, small-molecular-weight substances stay in the matrix for a longer time and move down more slowly, and are eluted after large-molecular-weight substances
Q3: Is this mounting medium water-soluble or lipid-soluble?
Water-soluble
Q4: This product is used for chemiluminescent immunoassay, similar to ELISA mode. Due to experimental needs, a lot of HRP is used, causing very strong light that quenches quickly. Can Solution A and B be diluted? With what? Which one should be diluted first?
Solution A and Solution B can be diluted with pure water. The ECL chemiluminescence kit is relatively large in size. The elisa mode attempt does not consume much reagent. Both Solution A and Solution B can be diluted.
Q5: The homology between human and mouse EGF is very low, only 55%. But you have verification data in mouse, so can it be used in mouse?
Yes, it's okay. This method is the one used in the pharmacopoeia and is fine. At present, this method is basically adopted in the industry.
Homology is not the only criterion for judgment. This is the case for all human cytokines with mouse data. Even if the homology is low, they can still be used.
Q6: For cytokines, is species important? Or if the sequence homology is high, is the difference small? I need mouse cytokines, but you only have human ones. Some activity data show use in mouse cells, others don't.
Theoretically, as long as the sequence homology is high, the difference in activity will not be significant. For those with low homology, the dosage used will vary among different species. For example, in this cell of L929 mice, refer to R& As for the data of D, human TNFa Its activity in mice is about 4 to 5 times weaker than that of murine TNF-a, meaning its effect is not as good as that of murine TNF-a.
Q7: Why do we need to verify in mouse cells? There's no Human data.
The main reason for using mouse cells for verification is that human cells are relatively difficult to purchase, and well-known domestic and foreign brands all conduct tests using mouse L929
Q8: For SP2/0 cell culture, does glutamine need to be added extra? Doesn't 1640 already contain glutamine?
Generally, 1640 medium is contained, but sometimes it may degrade during the storage process. For safety's sake, we suggest adding it
Q9: Can this tissue direct amplification kit be used for mouse cell samples?
It can be used for direct expansion of mouse tissue and cell samples
Q10: There are fine white particles in the 500ml Freund's Complete Adjuvant I bought. Should it be fine?
Under normal circumstances, there is a little sediment in the Freesier complete adjuvant because of mycobacteria. Just mix it thoroughly before use. The 10ml specification is relatively small, so it's not very obvious. The large package will be more obvious. Actually, both are available
Q11: What is the difference between Biodragon's Freund's Complete Adjuvant and Incomplete Adjuvant?
The difference between complete adjuvants and incomplete adjuvants is that complete adjuvants contain mycobacteria that are inactivated by ultrasound combined with water bath heating
Q12: What is the diameter of the gold particles in nanometers? Can it be used for electron microscopy?
25nm. I haven't tested an electron microscope
Q13: Can the QuickAntibody adjuvants for mice and rabbits be mixed? Can the mouse adjuvant be used for rabbits?
Rabbit adjuvants and mouse adjuvants should not be mixed. They are species-specific and it is not recommended to use them together
Q14: In literature, a common dose for Thermo's product is 2mg aluminum hydroxide in 50ul. Switching to Biodragon's, if I still need to inject 2mg aluminum hydroxide, how many ul are needed?
The following calculation methods can be referred to:
Thermo77161 is quantified at 40mg/ml of aluminium hydroxide (AI(OH) 3,78.004 g/mol), and after conversion, the concentration of aluminium ions (AI3*, 26.98g/mol) is 13.83mg/ml
② Aluminum ion content of Boaolong adjuvant :10mg/ml.
50 ulx13. 33 mg/mL; 10 mg/ml = 69.175 ul
Or, 2mgx26.98g/mol; 78.004 g/mol,; 10 mg/ml = 69.17 ul
That is, for 2mg of aluminium hydroxide, approximately 70 μ l of adjuvant is required
Q15: For mouse modeling, we recommend a dose of 50~100ug (aluminum ion per time). Literature reports using 60ul of Thermo product 77161, which converts to an aluminum ion concentration of 0.8mg per time. There's a big difference between the two dosages. How to choose?
The dosage provided in the instruction manual is the reference dose required for antibody preparation, not the modeling dose. Please explore the appropriate dosage based on the literature. The processes and ratios of adjuvants from different brands vary, so the reference dosages provided by different brands also differ. The common doses in the literature can be used as a reference starting point. Please adjust according to the evaluation results and determine the most suitable mouse strain, sensitization interval, allergen type and dose, adjuvant type and dose, etc.
Q16: Is it sterile or non-sterile?
Sterile aliquoted
Q17: What is the particle size of the aluminum hydroxide adjuvant?
Micrometer level
Q18: How many times can it be used?
The cross-sectional area of different tissues varies, and it is best to cover the sample, usually ranging from 50 to 100 μ l /Test, for Boao Long. The mIHC kit is a fluorescatically labeled Tyramide ready-to-use specification (1mL, 2mL, 5mL), meaning that 1mL corresponds to 10-20T of test samples, 2mL to 20-40T, and 5mL to 50-100T.
Q19: Q: I immunized mice with Freund's adjuvant, already 4 times, every 2 weeks. The measured titer seems mediocre. Now I want to change the immunogen and continue immunizing the same mouse to see if I can improve the titer. Which adjuvant is suitable and how to operate?
A: Previously, some customers tried to switch to our QuickAntibody. 5W adjuvant, administer two injections in a cycle as per the instructions. If you are really in a hurry, you can also try 30,000. The water adjuvant for two injections. However, the function of the immune system declines with age, so continuing immunization may increase the risk of inappropriate age for mice
Q20: Can it be used for subtype identification of recombinant monoclonal antibodies?
Our reagents are used to test hybridomas, not recombinant monoclonal antibodies. The subtype identification kit cannot be used for the detection of recombinant monoclonal antibodies. If it is cho or 293 expressed recombinant antibodies, they are not highly recommended. Recombinant antibodies do not guarantee a reaction
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